Volume 7, Issue 2, March 2018, Page: 20-24
Isolation, Identification and Antibiotic Susceptibility Pattern of Bacteria Isolated from Wounds of Patients Attending at Arsho Advanced Medical Laboratory
Messele Admassie, Department of Microbiology, Arsho Advanced Medical Laboratory, Addis Ababa, Ethiopia
Estifanos Tsige, Department of Microbiology, Arsho Advanced Medical Laboratory, Addis Ababa, Ethiopia
Meseret Chanie, Department of Microbiology, Arsho Advanced Medical Laboratory, Addis Ababa, Ethiopia
Received: Mar. 9, 2018;       Accepted: Apr. 2, 2018;       Published: May 11, 2018
DOI: 10.11648/j.sjcm.20180702.12      View  1516      Downloads  161
Wound infections cause clinical and societal consequences on the patients, but its bacteriological characteristicvaries with different factors. Therefore, effective treatment and management of wound infections in hospital and communitysetting will require detailed epidemiological knowledge of the infecting bacterial pathogens and their antibiogramunusual to theenvironment. Based on this information, we examined the frequency and antibiogram of bacterial pathogens isolated from woundinfection cases seen at ArshoAdvancedmedical laboratory over the study period. A total of 259 wound swabs/ and pus of different types of woundinfections from different anatomical sites were analyzed by standard bacteriological methods. Of the 259 clinical specimens analyzed, 177 (68.4%) yielded at least one bacterial pathogens, 6(2.3%) were polymicrobial, and 82(31.6%) yielded no bacterial growth. Overall, 20 different bacterial pathogens were identified 15 (75%) gram-negative bacteria isolates and 5(25%) gram-positive bacterial isolated. Staphylococcus aureus accounted formajority of the bacterial pathogens isolated, 86 (48.6% followed by E.coli20 (11.3%, and Citrobacterspp. 17(9.6%). The bacterial pathogens demonstrated high resistance to amoxicillin (79.7 %%), ampicillin (78.3%), and tetracycline (73.1%), in contrastto high sensitivity pattern observed with Meropinem (94.5%), Levofloxacin (87%), Amikacin (82.4%), and Ceftazidime (72.7%). Amikacin, meropenem and levofloxacin were the most effective drugs against the tested gram- positive and -negative bacteria and should be considered in empirical antibiotic selection.
Wound Infections, Bacterial Pathogens, Polymicrobial, Arsho
To cite this article
Messele Admassie, Estifanos Tsige, Meseret Chanie, Isolation, Identification and Antibiotic Susceptibility Pattern of Bacteria Isolated from Wounds of Patients Attending at Arsho Advanced Medical Laboratory, Science Journal of Clinical Medicine. Vol. 7, No. 2, 2018, pp. 20-24. doi: 10.11648/j.sjcm.20180702.12
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Esebelahie, N. O. Newton-Esebelahie, F. O. Omoregie R. Aerobic bacteria isolated from infected wound. African journal of clinical and expremental microbiology. 2013; 14(3):1595-689.
Egbe CA, Omoregie R, Igbarumah IO, Onemu S. Microbiology of wound infections and its associated risk factors among patients of a Tertiary hospital in Benin City, Nigeria. JRHS. 2011; 11(2):109-113.
Bowler PG, Duerden BI and Armstrong DG. Wound microbiology and associated approaches to wound management. Clinical Microbiology Review. 2001; 14:244-269.
Sibor JO, Oseni A, Eyaufe A, Osagie R. Turay A. Incidence of aerobic bacteria and Candida albicas in post operative wound infections. Afr J Microbiol Res. 2008; 2:288-291.
Aynalem Mohammed, Mengistu Endris Seid, Teklay Gebrecherkos, Moges Tiruneh, and Feleke Moges, “Bacterial Isolates and Their Antimicrobial Susceptibility Patterns of Wound Infections among Inpatients and Outpatients Attending the University of Gondar Referral Hospital, Northwest Ethiopia,” International Journal of Microbiology, 2017, 2017; 10.
Cheesbrough M. Microbiology: in Medical Laboratory Manual for Tropical countries. ELBS edition. University Press, Cambridge. 1999; 132: 26-58.
Bauer, A. W., W. M. Kirby, J. C. Sherris and M. Turck. Antibiotic susceptibility testing by a standard single discmethod. American Journal Clinical Pathology. 1966; 45:493-496.
Rao R, Basu R, Biswas DR. Aerobic Bacterial Profile and Antimicrobial Susceptibility Pattern ofPusIsolates in a South Indian Tertiary Care Hospital. IOSR-JDMS. 2014; 13(3):59-62.
Bularafa Mohammed Yasidiet al.: Retrospective Analysis of Bacterial Pathogens Isolated from Wound Infections at a Tertiary Hospital in Nguru, Yobe State Nigeria.
Khan I, Sarwar N, Ahmad B, Azam S, Rehman N Identification and Antimicrobial Susceptibility Profile of Bacterial Pathogens Isolated From Wound Infections in a Teaching Hospital, Peshawar, Pakistan. Adv. Life Sci. 2017; 5(1): 08-12.
Karia JB, Gadekar HB, Lakhani SJ. Study of bacterial profile of pus culture in dhirajGeneralhospital. Indian, J. SurgOncol. 2013; 4(2):172–218.
Shittu AO, Kolawole DO and Oyedepo EAR. A study ofwound infections in two health institutions in ile-ife, Nigeria. Afr. J. Biomed. Res. 2002; 5 (3): 97–102.
Muluye D, Wondimeneh Y, Ferede G, Nega T, Adane K, Biadgo B, Tesfa H, Moges. Bacterial isolates and their antibiotic susceptibility patterns among patients with pus and/or wound discharge at Gondar University hospital. BMC Research Notes. 2014; 7:619.
[14] Daniel SJC, Gowthani DE, Sowmiya S. Isolation and identification of bcateril pathogens from wound of diabetic patients. Int. J. Curr, Microbiol. Appl. Sci. 2013; 2(11):72-77.
Adane Bitew, Mesele Admassie, Tigist Getachew. Spectrum and Drug Susceptibility Profile of Bacteria Recovered from Patients with wound Infection Referred to Arsho Advanced Medical Laboratory. Clinical Medicine Research. 2018;7 (1): 8-17.
Manikandan C. and Amsath A. Antibiotic susceptibility of bacterial strains isolated from wound infection patients in Pattukkottai, Tamilnadu. India Int. J. Curr. Microbiol. App. Sci. 2013; 2(6): 195-203.
Syed AsadAli, S. M. Tahir, Abdul SattarMemon, Noshad A. Shaikh. Pattern of pathogens and their sensitivity isolated from superficial surgical site infections in a tertiary care hospital. JAyub Med Coll Abbottabad. 2009; 21(2).
Agwunglefah, F. D., Nwabunike, C. C. and Nwaju, P. C. Antibiotic Susceptibility Pattern of Bacteria Isolated From Surgical Wounds of Patients Attending Federal Medical Center and Christiana Specialist Hospital, Owerri. Journal of Natural Sciences Research. 2014; 4(15): 2224-3186.
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